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Updated: May 22, 2026

A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors
Published on: June 8, 2022
Assessment of sphingosine-1-phosphate activity in biological samples by receptor internalization and adherens
1Laboratory Medicine, Department of Pathology, Center for Vascular Biology, Weill Cornell Medical College, Cornell University, New York, NY, USA.
Abstract:
Sphingosine-1-phosphate (S1P) is a bioactive lipid mediator involved in many biological actions, including vascular homeostasis and immune cell trafficking. S1P activity is mediated by specific G protein-coupled receptors, leading to multiple physiological responses including adherens junction formation in endothelial cells. Here, we describe bioassays for rapidly assessing S1P activity in biological fluids based on ligand-induced receptor internalization in transfected HEK293 cells and consequent adherens junction formation of vascular endothelial cells.
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