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A room-temperature adenosine-based molecular beacon for highly sensitive detection of nucleic acids

Yen-Hsiu Lin1, Wei-Lung Tseng

  • 1Department of Chemistry, National Sun Yat-sen University, Kaohsiung, Taiwan.

Chemical Communications (Cambridge, England)
|April 26, 2012
PubMed

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...