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Related Experiment Video

Updated: May 22, 2026

Following the Dynamics of Structural Variants in Experimentally Evolved Populations

Following the Dynamics of Structural Variants in Experimentally Evolved Populations

Published on: February 3, 2023

Modelling the evolutionary dynamics of viruses within their hosts: a case study using high-throughput sequencing.

Frédéric Fabre¹, Josselin Montarry, Jérôme Coville

¹INRA, UR0407 Pathologie Végétale, Montfavet, France. frederic.fabre@avignon.inra.fr

|April 26, 2012

Summary

Mathematical models accurately describe virus evolution within hosts. This study reveals how natural selection and genetic drift influence Potato virus Y (PVY) dynamics, aiding in predicting virus emergence.

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Area of Science:

Virology
Evolutionary Biology
Population Genetics

Background:

Understanding virus evolution within hosts is crucial for predicting virus emergence.
Mathematical models are key tools for studying viral population dynamics and evolutionary trajectories.

Purpose of the Study:

To analyze the within-host population dynamics of Potato virus Y (PVY) variants using high-throughput sequencing.
To compare experimental results with six hypotheses on viral competitiveness and genetic drift.
To estimate the fitness effects of mutations and epistasis in PVY.

Main Methods:

High-throughput sequencing to analyze PVY variant frequencies within host plants.
Model selection procedures to evaluate hypotheses on viral competition and genetic drift.
Lotka-Volterra models and Dirichlet-multinomial distributions for data analysis.

Main Results:

PVY variant frequencies were well described by Lotka-Volterra models incorporating fitness ratios.
Specific mutations exhibited fitness costs (-0.45% to -13.2%) with negative epistasis.
Initial population size was small (1-4 infectious units per leaf).
Genetic differentiation among plants (FST) varied over time, peaking around 10 days post-inoculation.

Conclusions:

Mathematical models can accurately capture both selection and genetic drift in virus evolution.
The study provides insights into the evolutionary forces shaping PVY populations within plants.
Findings contribute to a better understanding of virus emergence and evolution.