Related Experiment Video
Updated: May 22, 2026

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach
Published on: December 4, 2021
Feasability of neat carbon dioxide packed column comprehensive two dimensional supercritical fluid chromatography
Pierre Guibal1, Didier Thiébaut, Patrick Sassiat
1UMR PECSA 7195 CNRS, UPMC, ESPCI, Laboratoire Sciences Analytiques, Bioanalytiques et Miniaturisation, ESPCI ParisTech, 10 rue Vauquelin, 75 005 Paris, France.
Abstract:
The design and implementation of comprehensive two dimensional supercritical fluid chromatography (SFC) using neat carbon dioxide as the mobile phase is described. Two conventional supercritical fluid chromatographs were hyphenated via an on line comprehensive 2D liquid chromatography like interface; it consisted of a two loop switching valve allowing the collection of the first dimension column effluent, the second dimension separation of a fraction being performed during the time allowed for the collection of the subsequent fraction of the first dimension eluent. Both dimension separations were monitored via UV detection; for the second dimension, the main flow was diverted to implement flame ionisation detection for the detection of hydrocarbons and the construction of the corresponding colour plots. Some key parameters related to the interfacing of the two dimensions and the chromatographic conditions used in both dimensions are discussed. In this preliminary report, the feasibility of comprehensive 2D SFC is demonstrated on synthetic mixtures of hydrocarbons and its potential on real sample analysis is illustrated by the separation of coal derived vacuum distillate.
Related Concept Videos
Supercritical Fluid Chromatography
SFC utilizes a supercritical fluid mobile phase,...
Principles Of Column Chromatography
Gas Chromatography: Types of Columns and Stationary Phases
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
Silica Gel Column Chromatography: Overview
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
Ion-Exchange Chromatography
Gas Chromatography: Introduction
In GC, a sample is vaporized and mixed with an inert carrier gas (the mobile phase), which transports it through a column.
