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Updated: May 22, 2026

Three-Dimensional Acoustic Assembly Device for Mass Manufacturing of Cell Spheroids
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Three-Dimensional Acoustic Assembly Device for Mass Manufacturing of Cell Spheroids

Published on: October 13, 2023

Controlled cell aggregation in a pulsed acoustic field.

Despina Bazou1, Angélica Castro, Mauricio Hoyos

  • 1Centre for Research on Adaptive Nanostructures and Nanodevices (CRANN), Trinity College, Dublin, Ireland.

Ultrasonics
|April 27, 2012
PubMed
Summary
This summary is machine-generated.

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Pulsed ultrasound offers precise control over cell and particle aggregation, enabling selective 2D or 3D aggregate formation. This method enhances control over aggregation rates and reduces transducer heating for higher acoustic energies.

Area of Science:

  • Acoustic manipulation
  • Biotechnology
  • Materials science

Background:

  • Cell and particle aggregation in ultrasonic resonators is rapid but difficult to control.
  • Existing methods lack precise control over aggregate configuration (2D/3D) and aggregation rate.

Purpose of the Study:

  • To introduce and validate a novel method for controlled generation of particle and cell aggregates using pulsed ultrasound.
  • To demonstrate precise control over aggregate configuration and aggregation rate through pulsed ultrasound parameters.

Main Methods:

  • Utilized pulsed ultrasound, varying parameters such as pulse number and repetition frequency.
  • Investigated the effects of these parameters on cell and particle levitation and aggregation.
  • Compared pulsed ultrasound with traditional continuous ultrasound methods.

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Fabrication and Operation of Acoustofluidic Devices Supporting Bulk Acoustic Standing Waves for Sheathless Focusing of Particles
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Microparticle Manipulation by Standing Surface Acoustic Waves with Dual-frequency Excitations
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Microparticle Manipulation by Standing Surface Acoustic Waves with Dual-frequency Excitations

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Related Experiment Videos

Last Updated: May 22, 2026

Three-Dimensional Acoustic Assembly Device for Mass Manufacturing of Cell Spheroids
05:17

Three-Dimensional Acoustic Assembly Device for Mass Manufacturing of Cell Spheroids

Published on: October 13, 2023

Fabrication and Operation of Acoustofluidic Devices Supporting Bulk Acoustic Standing Waves for Sheathless Focusing of Particles
10:14

Fabrication and Operation of Acoustofluidic Devices Supporting Bulk Acoustic Standing Waves for Sheathless Focusing of Particles

Published on: March 6, 2016

Microparticle Manipulation by Standing Surface Acoustic Waves with Dual-frequency Excitations
06:51

Microparticle Manipulation by Standing Surface Acoustic Waves with Dual-frequency Excitations

Published on: August 21, 2018

Main Results:

  • Pulsed ultrasound successfully levitates particles and cells, similar to continuous ultrasound.
  • Controlled modification of aggregation rates was achieved by tuning pulse parameters.
  • Selective generation of 2D and 3D aggregate configurations was demonstrated.
  • Pulsed ultrasound reduced transducer heating, allowing for higher acoustic energy application.

Conclusions:

  • Pulsed ultrasound provides a novel and highly controllable method for generating particle and cell aggregates.
  • Precise manipulation of aggregate morphology (2D/3D) and aggregation kinetics is achievable.
  • This technique offers advantages over continuous ultrasound, including improved control and reduced heating, enabling higher energy applications.