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Published on: April 11, 2016
A three-stage culture process for improved exopolysaccharide production by Tremella fuciformis
Hu Zhu1, Baozhen Tian, Wei Liu
1Center for Bioengineering and Biotechnology, China University of Petroleum, East China, Qingdao 266555, PR China. zhuhu@upc.edu.cn
Abstract:
Tremella fuciformis produces several bioactive secondary metabolites including exopolysaccharides. Cultivation of the fungus was carried out in a three-stage process consisting of a 1.5-day cultivation with orbital shaking at 200 rpm, a 1.5-day cultivation with reciprocal shaking at 200 strokes, and a 1.5-day cultivation with orbital shaking at 200 rpm. Exopolysaccharide production and specific production rate reached 5.80 g L(-1) and 0.15 d(-1), respectively, which is an increase of 260% and 200% compared with the corresponding values for fermentations with orbital shaking only, and of 243% and 150% compared with the corresponding values for fermentations with reciprocal shaking only. The three-stage culture method is time-saving and easy to operate.
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