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Updated: May 22, 2026

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

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Ultrafast pump-probe microscopy with high temporal dynamic range.

Matthias Domke¹, Stephan Rapp, Michael Schmidt

¹Munich University of Applied Sciences, Lothstrasse 34, 80335 Munich, Germany. matthias.domke@hm.edu

|April 27, 2012

Summary

This summary is machine-generated.

This study combines optical and electronic delays for high-resolution ultrafast pump-probe microscopy, enabling detailed observation of laser ablation processes from femtoseconds to microseconds. Researchers visualized molybdenum film ablation, revealing a laser lift-off mechanism.

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Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

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An Experimental Protocol for Femtosecond NIR/UV - XUV Pump-Probe Experiments with Free-Electron Lasers

An Experimental Protocol for Femtosecond NIR/UV - XUV Pump-Probe Experiments with Free-Electron Lasers

Published on: October 23, 2018

Area of Science:

Materials Science
Laser Physics
Surface Science

Background:

Ultrafast pump-probe microscopy is crucial for time-resolved imaging of laser ablation.
Existing methods use optical or electronic delays, limiting temporal resolution or range.
Observing complete ablation processes from femtoseconds to microseconds requires combined delay techniques.

Purpose of the Study:

To develop and demonstrate a combined optical and electronic delay system for ultrafast pump-probe microscopy.
To achieve high temporal resolution for observing complete laser ablation processes.
To investigate the substrate-side selective ablation of molybdenum films on glass.

Main Methods:

Combined optical and electronic delay lines for temporal control in pump-probe microscopy.
Developed methods for calibrating delay time zero point and synchronizing probe sources.
Implemented image quality enhancement techniques for improved visualization.

Main Results:

Achieved temporally high-resolved observations of laser ablation from femtoseconds to microseconds.
Successfully investigated the complete ablation process of molybdenum films on glass.
Observed mechanical film deformation initiating at ~400 ps, leading to a laser lift-off ablation at ~15 ns.

Conclusions:

The combined delay approach significantly enhances the capability of pump-probe microscopy for studying ultrafast phenomena.
Demonstrated a substrate-side selective laser lift-off ablation mechanism in molybdenum films, distinct from thermal effects.
Provides new insights into the dynamics of laser-induced material removal at the microscale.