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Updated: May 22, 2026

Manipulation of Single Neural Stem Cells and Neurons in Brain Slices using Robotic Microinjection
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Manipulation of Single Neural Stem Cells and Neurons in Brain Slices using Robotic Microinjection

Published on: January 21, 2021

High-throughput single-cell manipulation in brain tissue.

Joseph D Steinmeyer1, Mehmet Fatih Yanik

  • 1Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.

Plos One
|April 27, 2012
PubMed
Summary
This summary is machine-generated.

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This study introduces a high-throughput system for precise single-cell genetic manipulation in brain tissue. The automated platform enables efficient delivery of multiple reagents, advancing neuronal circuit analysis.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Biotechnology

Background:

  • Studying complex neuronal circuits requires precise single-cell genetic manipulation.
  • Current methods lack either single-cell accuracy or high throughput and repeatability.

Purpose of the Study:

  • To develop a high-throughput, reliable system for combinatorial genetic vector and reagent delivery into single cells within brain tissue.
  • To enable precise manipulation and tracking of neuronal activity at single-cell resolution.

Main Methods:

  • An automated system for nanoliter-scale reagent loading from multiwell plates into a micropipette.
  • A robust electroporation technique for targeted single-cell transfection in brain tissue (organotypic and acute slices).
  • Automated micropipette cleaning for repeated transfection cycles.

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Single Cell Electroporation in vivo within the Intact Developing Brain
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Single Cell Electroporation in vivo within the Intact Developing Brain

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Preparation of Neuronal Co-cultures with Single Cell Precision
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Preparation of Neuronal Co-cultures with Single Cell Precision

Published on: May 20, 2014

Related Experiment Videos

Last Updated: May 22, 2026

Manipulation of Single Neural Stem Cells and Neurons in Brain Slices using Robotic Microinjection
11:40

Manipulation of Single Neural Stem Cells and Neurons in Brain Slices using Robotic Microinjection

Published on: January 21, 2021

Single Cell Electroporation in vivo within the Intact Developing Brain
13:31

Single Cell Electroporation in vivo within the Intact Developing Brain

Published on: July 11, 2008

Preparation of Neuronal Co-cultures with Single Cell Precision
09:06

Preparation of Neuronal Co-cultures with Single Cell Precision

Published on: May 20, 2014

Main Results:

  • Demonstrated high-throughput, efficient, and reliable combinatorial delivery of multiple genetic vectors.
  • Achieved multi-colored labeling of adjacent cells and transfection of different protein isoforms into single neurons.
  • Successfully analyzed effects on dendritic spine density and demonstrated potential for in vivo and ex vivo applications.

Conclusions:

  • The developed platform offers unprecedented throughput and accuracy for single-cell genetic manipulation in neuroscience research.
  • This technology facilitates detailed analysis of neuronal circuit function and manipulation of neuronal activity.
  • Potential applications include delivery of optogenetic tools, cell-type specific agents, and various sensors for real-time monitoring.