Related Experiment Videos
Approaches to measurement of vitamin D concentrations - mass spectrometry
1Roche Diagnostic GmbH, Nonnenwald 2, D-82377 Penzberg - Germany. uwe.kobold@roche.com
Abstract:
Mass spectrometry today is the only analytical technology which allows the specific determination of all known vitamin D metabolites. During the last few years the number of published methods rapidly increased and quantitative HPLC-MS/MS procedures are described for all the major metabolites including 25(OH)D3, 3-epi25(OH)D3, 24,25(OH)(2)D3, 25(OH)D2 and 1,25(OH)(2)D3. For the first time these new methods have made the systematic study of the clinical relevance of vitamin D metabolites possible. In parallel to the development of methods for new metabolites, significant progress was made in improving the performance of HPLC-MS/MS for quantifying 25(OH)D3 concentrations which has resulted in very short run times and thus enabling high throughput analysis for routine use and large sample sets.
Related Concept Videos
Tandem Mass Spectrometry
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
MALDI-TOF Mass Spectrometry
Mass Spectrometry: Overview
Mass Spectrometers
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...