Related Experiment Video
Updated: May 22, 2026
09:06
Preparation of Neuronal Co-cultures with Single Cell Precision
Published on: May 20, 2014
Formation of one-way-structured cultured neuronal networks in microfluidic devices combining with micropatterning
Yuzo Takayama1, Naoki Kotake, Tatsuya Haga
1Graduate School of Information Science and Technology, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. yuzo_takayama@ipc.i.u-tokyo.ac.jp
Journal of Bioscience and Bioengineering
|April 28, 2012
Abstract:
We present a simple method to regulate the direction of axon development in cultured neurons using microfabrication and microfluidics techniques. We fabricate a PDMS-based device and place it onto a chemically micropatterned glass substrate. We confirm that cultured neurons extend neurites along the medium flow direction and the micropatterned regions.
