Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Chromatin Immunoprecipitation- ChIP

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...

Immunoprecipitation

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Hnrnpa1 is essential for early zebrafish development and lipid metabolism: insights from a novel zebrafish knockout model.

Frontiers in cell and developmental biology·2026

Same author

A spatial in situ hybridization approach to T cell clonotype analysis using T cell receptor variable gene probes.

Cell reports·2026

Same author

Amyloid precursor protein ortholog Appl acts with Vnd during mushroom body axon growth in Drosophila.

Genetics·2026

Same author

Integrative omics and phase IIa clinical trial identify TNF as key node in autoimmune hepatitis.

Journal of hepatology·2026

Same author

Encephalopathy-linked UFM1 variants impede neuronal protein translation, development, and function.

EMBO molecular medicine·2026

Same author

Re: Qi et al. "A roadmap for T cell receptor-peptide-MHC binding prediction by machine learning: glimpse and foresight" (Briefings in Bioinformatics, 2025).

Briefings in bioinformatics·2026

Same journal

High-throughput measurements of protein domain functions using magnetic separation.

Nature protocols·2026

Same journal

Inducing physiological polarity and performing gene editing using CRISPR-Cas9 in human trophoblast organoids.

Nature protocols·2026

Same journal

Photocatalytic low-temperature defluorination of PTFE.

Nature protocols·2026

Same journal

Multimodal imaging and quantification of lanthanide chelate-labeled micro- and nanoplastics in plants.

Nature protocols·2026

Same journal

Facilitating structure-based drug discovery with an artificial intelligence-driven virtual screening platform.

Nature protocols·2026

Same journal

Yeast nuclei-mediated precise delivery of synthetic megabase-scale human DNA into mammalian embryos.

Nature protocols·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 22, 2026

TChIP-Seq: Cell-Type-Specific Epigenome Profiling

TChIP-Seq: Cell-Type-Specific Epigenome Profiling

Published on: January 23, 2019

Cell type-specific chromatin immunoprecipitation from multicellular complex samples using BiTS-ChIP.

Stefan Bonn¹, Robert P Zinzen, Alexis Perez-Gonzalez

¹Genome Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.

Nature Protocols

|April 28, 2012

Summary

This summary is machine-generated.

This new protocol enables batch isolation of tissue-specific chromatin for precise analysis of gene regulation. It uses formaldehyde cross-linking and FACS sorting for high-purity chromatin, applicable across model systems.

More Related Videos

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing (ChIP-seq)

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing (ChIP-seq)

Published on: April 19, 2013

Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples

Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples

Published on: August 29, 2017

Related Experiment Videos

Last Updated: May 22, 2026

TChIP-Seq: Cell-Type-Specific Epigenome Profiling

TChIP-Seq: Cell-Type-Specific Epigenome Profiling

Published on: January 23, 2019

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing (ChIP-seq)

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing (ChIP-seq)

Published on: April 19, 2013

Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples

Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples

Published on: August 29, 2017

Area of Science:

Molecular Biology
Genomics
Epigenetics

Background:

Analyzing gene regulation in multicellular organisms requires isolating specific cell types.
Existing methods for chromatin isolation can lead to loss of transcriptional context or purity.

Purpose of the Study:

To describe a novel protocol for batch isolation of tissue-specific chromatin (BiTS-ChIP).
To enable analysis of histone modifications, transcription factor binding, and polymerase occupancy.
To provide a method applicable to various model systems, optimized for Drosophila.

Main Methods:

Formaldehyde cross-linking of embryos expressing cell type-specific nuclear markers.
Dissociation, isolation, and Fluorescence-Activated Cell Sorting (FACS) of nuclei based on marker expression.
Extraction and sonication of tissue-specific chromatin for downstream analyses like ChIP-seq.

Main Results:

The BiTS-ChIP method preserves transcriptional context by cross-linking before dissociation.
FACS sorting of nuclei yields highly pure, tissue-specific chromatin.
The complete protocol, including library preparation, can be finished within 5 days.

Conclusions:

BiTS-ChIP is an efficient and high-purity method for isolating tissue-specific chromatin.
This protocol facilitates detailed epigenomic and transcriptomic analyses in complex tissues.
The method's adaptability makes it valuable for diverse model organisms in biological research.