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Construction of a promoter-probe shuttle vector for Escherichia coli and brevibacteria
I Barák1, M Koptides, M Jucovic
1Department of Recombinant DNA Technology, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Gene
|October 30, 1990
Abstract:
We constructed a promoter-probe vector, pJUP05, for brevibacteria and Escherichia coli based on the promoterless neomycin-resistance (neoR) gene from Tn5. This gene confers resistance to the aminoglycosides, kanamycin and neomycin. The promoter of the neoR gene was deleted and replaced by a suitable multiple cloning site. There are translation stop codons in all three reading frames upstream from the neoR gene. The plasmid contains functional origins of DNA replication for both brevibacteria and E. coli, and permits selection for chloramphenicol- and/or ampicillin-resistance markers.