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Calf thymus DNA binding studies of the new neodymium-naproxen complex

Ruina Huo¹, Guiqing Xu, Xiaoying Jiang

¹School of Chemistry and Environmental Sciences, Henan Normal University, Xinxiang 453007, People's Republic of China.

Journal of Biochemical and Molecular Toxicology

|April 28, 2012

Abstract:

Fluorescence spectroscopy in combination with UV absorption spectroscopy was carried out to investigate the interaction between the neodymium-naproxen complex (Nd-NAP) and calf thymus DNA (ctDNA). The experimental results showed that Nd-NAP intercalated with the ctDNA base pairs. Analysis of fluorescence quenching data of Nd-NAP by ctDNA at different temperatures using a Stern-Volmer equation revealed that dynamic and static quenching occurred simultaneously. The binding constants and the number of binding sites at 293 and 310 K were obtained as 2.904 × 10(4) L mol(-1), 1.172 and 2.432 × 10(4) L mol(-1), 1.143, respectively. The thermodynamic parameters ΔG, ΔH, and ΔS calculated at different temperatures indicated that hydrogen bonding and van der Waals force were the main binding forces.

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