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Updated: May 22, 2026
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Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
Published on: November 1, 2019
Stepping towards highly flexible aptamers: enzymatic recognition studies of unlocked nucleic acid nucleotides
Camille Dubois1, Meghan A Campbell, Stacey L Edwards
1School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Brisbane, 4072, Australia.
Chemical Communications (Cambridge, England)
|April 28, 2012
Abstract:
Enzymatic recognition of unlocked nucleic acid (UNA) nucleotides was successfully accomplished. Therminator DNA polymerase was found to be an efficient enzyme in primer extension reactions. Polymerase chain reaction (PCR) amplification of a 81 mer UNA-modified DNA library was efficiently achieved by KOD DNA polymerase.
