Related Experiment Video
Updated: May 22, 2026
09:50
Detection of Tissue-resident Bacteria in Bladder Biopsies by 16S rRNA Fluorescence In Situ Hybridization
Published on: October 18, 2019
Multiphoton microscopy in the evaluation of human bladder biopsies
Manu Jain1, Brian D Robinson, Douglas S Scherr
1Department of Urology, Weill Cornell Medical College, 1300 York Avenue, NewYork, NY 10065, USA.
Archives of Pathology & Laboratory Medicine
|May 1, 2012
Summary
Multiphoton microscopy (MPM) accurately diagnosed bladder cancer in 88% of cases, showing high sensitivity (90.4%) and specificity (76.9%). This advanced imaging technique offers potential for early bladder cancer detection.
Area of Science:
- Biomedical Imaging
- Optical Microscopy
- Uropathology
Background:
- Multiphoton microscopy (MPM) is a nonlinear imaging technique that provides cellular and subcellular details from unprocessed tissue by exciting intrinsic emissions.
- MPM is an emerging technology with potential clinical applications due to anticipated miniaturization and cost reduction.
Purpose of the Study:
- To evaluate the imaging capabilities of MPM for human bladder biopsies.
- To determine the diagnostic accuracy of MPM in classifying bladder lesions as benign or neoplastic.
Main Methods:
- Seventy-seven fresh bladder biopsies were imaged using MPM.
- MPM images were compared to routine surgical pathology diagnoses (hematoxylin-eosin stained sections).
- Twelve cases with extensive cautery artifact were excluded.
Main Results:
- Accurate MPM diagnoses (benign or neoplastic) were achieved in 88% of evaluable cases (57/65).
- MPM demonstrated a sensitivity of 90.4% and specificity of 76.9% for detecting neoplastic lesions.
- Architecture was correctly determined in 86% of cases, and cytologic grade in 68%.
Conclusions:
- MPM images alone provide sufficient detail to classify bladder lesions using standard histopathological criteria (architecture and cytologic grade).
- MPM shows promise as a tool for urologists and pathologists in the early detection of bladder cancer.
- Further exploration of MPM applications in diagnostics is warranted.
