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Published on: June 7, 2016
Expression of microRNAs in basal cell carcinoma
1Department of Dermatology, Venereology and Allergology, Ruhr-University Bochum, 44791 Bochum, Germany. michael.sand@ruhr-uni-bochum.de
The British Journal of Dermatology
|May 1, 2012
Summary
This study identified 16 upregulated and 10 downregulated microRNAs (miRNAs) in basal cell carcinoma (BCC). These findings suggest potential roles for these miRNAs in BCC development and progression.
Area of Science:
- Oncology
- Molecular Biology
- Dermatology
Background:
- MicroRNA (miRNA) expression profiles are altered in various cancers.
- Systematic evaluation of differentially expressed miRNAs in basal cell carcinoma (BCC) is lacking.
Purpose of the Study:
- To conduct a microarray-based miRNA profiling study.
- To identify specific miRNA candidates differentially expressed in BCC.
Main Methods:
- Microarray analysis of miRNA expression profiles from 7 BCC tumor samples and 7 adjacent nonlesional skin controls.
- Screening of 1205 Homo sapiens (hsa)-miRNA candidates using miRBase 16.
- Validation of seven dysregulated miRNAs using TaqMan quantitative real-time reverse transcription polymerase chain reaction.
Main Results:
- Identified 16 significantly upregulated miRNAs in BCC compared to nonlesional skin.
- Identified 10 significantly downregulated miRNAs in BCC compared to nonlesional skin.
- Detected associations between dysregulated miRNAs and tumor-promoting pathways like Hedgehog and MAPK/ERK signaling.
Conclusions:
- Several miRNA candidates were identified as potentially playing a role in the molecular pathogenesis of BCC.
- These findings provide a basis for further investigation into miRNA functions in BCC.
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