Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Recombinant DNA01:09

Recombinant DNA

Overview
Recombinant DNA01:09

Recombinant DNA

Overview
Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
Vaccine Production01:23

Vaccine Production

Vaccine production involves a sequence of upstream and downstream processes to generate a safe and effective immunological product. It begins with cultivating microorganisms, such as viruses or bacteria, to obtain antigenic material. For viral vaccines, mammalian host cells are grown in bioreactors and subsequently infected with the target virus. The virus replicates within the host cells, which are lysed to release viral particles. This lysate is then clarified through filtration or...
Vaccines01:21

Vaccines

Vaccines are among the most effective tools in preventive medicine, designed to prepare the immune system to recognize and combat infectious agents. By introducing antigens—substances that the immune system identifies as foreign—vaccines stimulate an adaptive immune response that leads to immunological memory. This immunological memory enables the body to mount a faster and more effective response upon future exposures to the actual pathogen.Vaccines can be categorized based on the type of...
Bacterial Transformation01:33

Bacterial Transformation

In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Microbial-Responsive Wound Dressings Based on Biopolymer Degradation Strategy for Detecting Bacterial Infections.

ACS applied materials & interfaces·2026
Same author

The staphylococcal type VII secretion system protein EsxC impacts daptomycin sensitivity through controlling bacterial cell envelope integrity.

Journal of bacteriology·2026
Same author

Smart antimicrobial wound dressings based on mechanically and biologically tuneable hybrid films.

Journal of materials chemistry. B·2025
Same author

Unravelling three-way interactions between <i>Clostridioides difficile</i>, microbiota and the host.

Journal of medical microbiology·2025
Same author

A novel GMMA-based gonococcal vaccine demonstrates functional immune responses in mice.

NPJ vaccines·2025
Same author

Erratum for Hassall et al., "Dissecting Individual Interactions between Pathogenic and Commensal Bacteria within a Multispecies Gut Microbial Community".

mSphere·2024

Related Experiment Video

Updated: May 22, 2026

Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria
06:38

Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria

Published on: December 8, 2023

Recombinant bacterial vaccines.

Meera Unnikrishnan1, Rino Rappuoli, Davide Serruto

  • 1Novartis Vaccines and Diagnostics, Via Fiorentina 1, 53100, Siena, Italy.

Current Opinion in Immunology
|May 1, 2012
PubMed
Summary

Vaccines have greatly improved global public health, but new vaccines are needed for emerging diseases. Advanced technologies like recombinant DNA are revolutionizing vaccine development for safer, more effective options.

Area of Science:

  • Immunology and Microbiology
  • Biotechnology and Genetic Engineering

Background:

  • Vaccination is a cornerstone of global public health, significantly reducing infectious disease morbidity and mortality.
  • Existing vaccines primarily utilize live attenuated or inactivated microbial agents.
  • There remains a critical need for novel vaccines against numerous infectious diseases, including those caused by emerging pathogens and for replacing suboptimal existing vaccines.

Purpose of the Study:

  • To highlight the limitations of current vaccine technologies.
  • To emphasize the need for new and improved vaccine candidates.
  • To introduce the transformative potential of modern biotechnological approaches in vaccine development.

Main Methods:

  • Review of current vaccine technologies and their limitations.

More Related Videos

Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection
12:09

Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection

Published on: May 2, 2011

Related Experiment Videos

Last Updated: May 22, 2026

Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria
06:38

Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria

Published on: December 8, 2023

Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection
12:09

Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection

Published on: May 2, 2011

  • Exploration of advancements in recombinant DNA technology.
  • Integration of genomics and structural biology in vaccine design.

    • Main Results:

    • Current vaccine strategies predominantly rely on traditional methods like live attenuated or inactivated pathogens.
    • A significant gap exists in vaccine coverage for many infectious diseases.
    • Emerging technologies offer unprecedented opportunities for designing next-generation vaccines.

    Conclusions:

    • Novel approaches, including recombinant DNA technology, genomics, and structural biology, are revolutionizing vaccine candidate development.
    • These advanced technologies promise to significantly enhance the safety and efficacy of future vaccines.
    • The continued development of innovative vaccine strategies is crucial for addressing unmet public health needs and combating infectious diseases.