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MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
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Amplification and up-regulation of microRNA-30b in oral squamous cell cancers

Chenying Shao1, Yili Yu, Liying Yu

  • 1Department of Stomatology, Huashan Hospital, Fudan University, Shanghai 200040, China.

Archives of Oral Biology
|May 1, 2012
PubMed
Abstract

Insights

Copy number gains in the MIR30B gene are common in advanced oral squamous cell cancers (OSCCs) and link to increased microRNA-30b (hsa-miR-30b) expression, suggesting a role in OSCC progression.

Area of Science:

  • Genomics
  • Molecular Biology
  • Oncology

Background:

  • MicroRNAs (miRNAs) are key regulators of gene expression, often dysregulated in cancers.
  • Overexpression of microRNA-30b (hsa-miR-30b) is linked to tumor invasion and immunosuppression.
  • The MIR30B gene locus (8q24) is frequently amplified in oral squamous cell cancers (OSCCs).

Purpose of the Study:

  • To investigate copy number variations (CNVs) of the MIR30B gene in OSCCs.
  • To analyze MIR30B gene expression levels in OSCCs.
  • To correlate MIR30B CNVs and expression with OSCC tumor stage.

Main Methods:

  • Quantitative real-time PCR used to assess MIR30B gene copy number and hsa-miR-30b expression.
  • 107 OSCC samples and matched adjacent normal tissues analyzed.
  • Proportional odds regression and ANOVA used for statistical analysis of CNVs and expression.

Main Results:

  • MIR30B gene copy number gains detected in 27.1% of OSCC samples.
  • MIR30B gene amplification significantly correlated with advanced tumor stages (p<0.001).
  • Gene amplification strongly associated with hsa-miR-30b overexpression (p<0.001), but overexpression also occurred with normal copy numbers.

Conclusions:

  • Increased MIR30B gene copy number is frequent in advanced OSCC.
  • MIR30B gene amplification correlates with hsa-miR-30b overexpression in OSCC.
  • OSCCs may utilize diverse regulatory mechanisms for MIR30B.

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