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Polymorphism in Brucella spp. due to highly repeated DNA.

S M Halling¹, E S Zehr

¹U.S. Department of Agriculture, National Animal Disease Center, Ames, Iowa 50010.

Journal of Bacteriology

|December 1, 1990

Summary

Brucella ovis lacks the common BCSP31 protein due to a DNA insertion downstream of its gene. This insertion, present in all tested B. ovis strains, differs significantly from Brucella abortus, impacting gene regulation.

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Area of Science:

Microbiology
Genetics
Molecular Biology

Background:

Brucella species are closely related, yet Brucella ovis lacks the common BCSP31 protein.
Understanding genetic variations is crucial for Brucella species differentiation.

Purpose of the Study:

Investigate the genetic basis for the absence of BCSP31 expression in Brucella ovis.
Characterize the genetic differences between Brucella ovis and other Brucella species.

Main Methods:

Southern blot analysis using a Brucella abortus BCSP31 gene probe.
DNA hybridization and restriction mapping of Brucella ovis strains.
Comparative genomic analysis between Brucella ovis and Brucella abortus.

Main Results:

The BCSP31 gene is present in Brucella ovis but located on a larger HindIII fragment (1.6 kb vs. 1.3 kb).
A DNA insertion of approximately 0.9 kb downstream of the BCSP31 coding region causes this polymorphism in B. ovis.
Significant differences in the number of repeated DNA fragments were observed between B. ovis (at least 24) and B. abortus (6).

Conclusions:

A downstream DNA insertion explains the lack of BCSP31 expression in Brucella ovis.
The observed genomic polymorphism, particularly the repeated DNA elements, distinguishes Brucella ovis from Brucella abortus despite their close evolutionary relationship.

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