An improved medium for growing Staphylococcus aureus biofilm
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Updated: May 22, 2026

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Targeting Biofilm Associated Staphylococcus aureus Using Resazurin Based Drug-susceptibility Assay
Published on: May 5, 2016
Ping Chen1, Johnathan J Abercrombie, Nicole R Jeffrey
1Microbiology Branch, US Army Dental and Trauma Research Detachment, Institute of Surgical Research, Fort Sam Houston, TX 78234, USA. ping.chen@us.army.mil
Journal of Microbiological Methods
|May 1, 2012
Abstract:
A medium (Brain Heart Infusion plus 10% human plasma) was developed, tested, and validated for growing Staphylococcus aureus biofilm in vitro. With this medium, S. aureus forms reproducible and robust biofilms in flow chambers under controlled shear flow and with increased viability recovery in static well plates.
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