Related Experiment Videos
Mechanism of enzymatic dehalogenation of pentachlorophenol by Arthrobacter sp. strain ATCC 33790
T Schenk1, R Müller, F Lingens
1Institut für Mikrobiologie, Universität Hohenheim, Stuttgart, Federal Republic of Germany.
Journal of Bacteriology
|December 1, 1990
Abstract:
Pentachlorophenol (PCP) dehalogenase from Arthrobacter sp. strain ATCC 33790 converts PCP to tetrachlorohydroquinone. In labeling experiments with H(2)18O or 18O2, only with H(2)18O was labeled product found. However, unlabeled tetrachlorohydroquinone became labeled after incubation with the enzyme in H(2)18O. Therefore, distinction between an oxygenolytic or a hydrolytic dehalogenation mechanism for the PCP dehalogenase is not possible.