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Related Concept Videos

Neural Regulation01:37

Neural Regulation

Digestion begins with a cephalic phase that prepares the digestive system to receive food. When our brain processes visual or olfactory information about food, it triggers impulses in the cranial nerves innervating the salivary glands and stomach to prepare for food.
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...

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A regulatory module controlling pharyngeal development and function in Caenorhabditis elegans.

David S Fay1, Stanley R G Polley, Jujiao Kuang

  • 1Department of Molecular Biology, College of Agriculture and Natural Resources, University of Wyoming, Laramie, WY 82071, USA. davidfay@uwyo.edu

Genetics
|May 1, 2012
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Summary

SUP-37, a novel protein, regulates Caenorhabditis elegans foregut development by controlling pha-1 gene expression. It also plays essential roles in muscle contraction for pumping and ovulation.

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Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • Foregut development in Caenorhabditis elegans involves transcriptional regulators and signaling pathways.
  • PHA-1 is an essential cytoplasmic protein with an unknown function in this process.
  • LIN-35 and UBC-18-ARI-1 pathways regulate pha-1 and pharyngeal development via SUP-35/ZTF-21.

Purpose of the Study:

  • To characterize SUP-37/ZTF-12 as a component of the PHA-1 regulatory network.
  • To investigate the role of SUP-37 in pharyngeal development and its relationship with SUP-35.
  • To identify additional functions of SUP-37 beyond pharyngeal development.

Main Methods:

  • Genetic analysis of sup-37 loss-of-function mutations.
  • Molecular characterization of SUP-37 splice isoforms and localization.
  • Expression analysis and genetic interaction studies.

Main Results:

  • SUP-37, a nuclear protein with multiple Zn-finger domains, regulates pharyngeal development.
  • SUP-37 loss-of-function mutations suppress pha-1 and lin-35; ubc-18 synthetic-lethal mutations.
  • SUP-37 and SUP-35 likely act together in pha-1 transcriptional regulation, bypassing pha-1 activity requirement.
  • SUP-37 functions independently of LIN-35 and UBC-18-ARI-1 pathways in pharyngeal development.
  • SUP-37 is essential for coordinated muscle contraction in pharyngeal pumping and ovulation.

Conclusions:

  • SUP-37 is a novel regulator of Caenorhabditis elegans pharyngeal development, acting in a network with SUP-35 and potentially regulating pha-1 transcription.
  • SUP-37 has essential roles in muscle function for coordinated contraction in multiple tissues, distinct from its role in pharyngeal development.