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CA-125 secretion by luteal phase endometrium in vitro
J R Brumsted1, J H McBean, J L Deaton
1Department of Obstetrics and Gynecology, University of Vermont College of Medicine, Burlington 05405.
Human Reproduction (Oxford, England)
|August 1, 1990
Summary
The endometrium secretes CA-125, a marker influenced by hormones. Progesterone (P) suppresses endometrial CA-125 secretion, which increases from the early to late luteal phase.
Area of Science:
- Gynecology
- Reproductive Endocrinology
- Biochemistry
Background:
- The origin of CA-125 in healthy women and its regulatory mechanisms are not fully understood.
- CA-125 is a biomarker often monitored in gynecological conditions.
Purpose of the Study:
- To investigate the secretion pattern of CA-125 from the endometrium during the luteal phase.
- To determine the influence of progesterone and oestradiol on endometrial CA-125 production.
Main Methods:
- Endometrial samples were collected from women in the early (days 16-18) and late (days 25-27) luteal phases.
- Tissue explants were cultured with no additions (NA), progesterone (P), oestradiol (E2), or a combination of E2 + P.
- CA-125 concentrations in culture media were measured using immunoradiometric assay.
Main Results:
- Endometrial CA-125 secretion was significantly higher in NA conditions compared to P or E2 + P treatments in both early and late luteal phases.
- CA-125 concentrations were higher in the late luteal phase compared to the early luteal phase across all treatment groups.
- Progesterone demonstrated a suppressive effect on endometrial CA-125 secretion.
Conclusions:
- The endometrium is a potential source of serum CA-125.
- Endometrial CA-125 secretion is suppressed by progesterone during both early and late luteal phases.
- Endometrial CA-125 secretion increases from the early to the late luteal phase.