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Larval RNA Interference in Silkworm Bombyx mori through Chitosan/dsRNA Nanoparticle Delivery
Published on: October 4, 2024
A 50-kilodalton Cry2A peptide is lethal to Bombyx mori and Lymantria dispar
Masataka Ohsawa1, Miki Tanaka, Kenta Moriyama
1Laboratory of Applied Biochemistry, Graduate School of Science and Technology, Niigata University, Japan.
Applied and Environmental Microbiology
|May 1, 2012
Abstract:
The Cry2Aa3 gene was introduced into asporogenic Bacillus thuringiensis, and the synthesized protoxin killed Bombyx mori and Lymantria dispar larvae. Chymotrypsin hydrolyzed the linkages between 49Tyr/Val50 and 145Lys/Ser146 in the protoxin, and 50- and 58-kDa fragments were generated, respectively. Both peptides killed the larvae of both insects.

