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Optimizing parameters for correlative immunogold localization by video-enhanced light microscopy, high-voltage
S R Simmons1, J B Pawley, R M Albrecht
1Department of Veterinary Science, University of Wisconsin, Madison 53706.
Abstract:
Correlative video-enhanced light microscopy, high-voltage transmission electron microscopy, and low-voltage high resolution scanning electron microscopy were used to examine the binding of colloidal gold-labeled fibrinogen to platelet surfaces. Optimal conditions for the detection of large (18 nm) and small (3 nm) gold particles are described.