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Updated: May 22, 2026
Partial Sciatic Nerve Ligation: A Mouse Model of Chronic Neuropathic Pain to Study the Antinociceptive Effect of Novel Therapies
Published on: October 6, 2022
Ameliorative effects of standardized extract from Trigonella foenum-graecum L. seeds on painful peripheral neuropathy
Aashish S Morani1, Subhash L Bodhankar, V Mohan
1Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Erandwane, Paud Road, Pune, India.
Objective:
To evaluate the effects of the standardized extract of fenugreek (Trigonella foenum-graecum L. Family: Leguminasae) seed (IND01) in animal models of peripheral neuropathy.
Methods:
IND01 was prepared from fenugreek seeds and standardized by high performance liquid chromatography to a marker compound, trigonelline. The effects of daily oral administration of IND01 (50, 100 and 200 mg/kg) were studied in rats after partial sciatic nerve ligation (PSNL) and sciatic nerve crush injury (SNCI) during 30-days period. The measurements on thermal hyperalgesia (TH), motor function test (MFT) score and motor nerve conduction velocity (MNCV) were recorded.
Results:
IND01 offered sustained protection against TH and deranged MFT scores in both models from 7-day onwards. Fifteen days of daily oral administration of IND01 restored MNCV reduction in rats with SNCI but not with PSNL.
Conclusions:
IND01 was found to be effective in rat models of painful peripheral neuropathy.
