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Related Concept Videos

Telomeres and Telomerase02:41

Telomeres and Telomerase

In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded DNA.
Telomeres and Telomerase02:41

Telomeres and Telomerase

In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded DNA.
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replicative Cell Senescence02:15

Replicative Cell Senescence

Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds the telomeric...

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Related Experiment Video

Updated: May 22, 2026

Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol (TRAP) Assay
10:14

Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol (TRAP) Assay

Published on: September 2, 2014

Assays for human telomerase activity: progress and prospects.

Xiaoming Zhou1, Da Xing

  • 1MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.

Chemical Society Reviews
|May 2, 2012
PubMed
Summary

Human telomerase, an enzyme linked to cancer, requires advanced detection methods. This review explores new bioanalytical techniques for identifying telomerase activity and inhibition, crucial for cancer diagnostics and therapeutics.

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Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence
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Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence

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Related Experiment Videos

Last Updated: May 22, 2026

Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol (TRAP) Assay
10:14

Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol (TRAP) Assay

Published on: September 2, 2014

Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein
08:26

Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein

Published on: June 12, 2018

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence
12:08

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence

Published on: May 22, 2013

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Oncology

Background:

  • Human telomerase is a ribonucleoprotein enzyme essential for maintaining telomere length.
  • Telomerase activity is strongly associated with cancer development and progression.
  • Accurate detection of telomerase is vital for cancer diagnostics, prognostics, and therapeutic targeting.

Purpose of the Study:

  • To review and highlight recent advancements in bioanalytical methods for detecting telomerase activity.
  • To discuss challenges and future directions in the development of innovative telomerase assays.
  • To summarize current technologies for telomerase testing in clinical and research settings.

Main Methods:

  • Review of current literature on bioanalytical methods for telomerase detection.
  • Analysis of novel techniques for assessing telomerase activity and inhibition.
  • Discussion of assay design challenges and technological limitations.

Main Results:

  • Several new methods offer rapid, sensitive, and reliable detection of telomerase activity.
  • Existing techniques face challenges in specificity, throughput, and clinical applicability.
  • Emerging technologies show promise for improved telomerase testing.

Conclusions:

  • Advancements in bioanalytical methods are crucial for leveraging telomerase as a cancer biomarker and therapeutic target.
  • Overcoming assay design challenges will enhance the clinical utility of telomerase testing.
  • Future research should focus on developing more robust and versatile telomerase detection platforms.