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Related Experiment Video

Updated: May 22, 2026

High-throughput, Microscale Protocol for the Analysis of Processing Parameters and Nutritional Qualities in Maize (Zea mays L.)

High-throughput, Microscale Protocol for the Analysis of Processing Parameters and Nutritional Qualities in Maize (Zea mays L.)

Published on: June 16, 2018

A high-throughput core sampling device for the evaluation of maize stalk composition.

German Muttoni¹, James M Johnson, Nicholas Santoro

¹Department of Agronomy, University of Wisconsin, 1575 Linden Drive, Madison, WI, 53706, USA. ndeleongatti@wisc.edu.

Biotechnology for Biofuels

|May 3, 2012

Summary

A new mechanical maize stalk core sampling device (CSD) enables rapid, high-throughput collection of uniform biomass samples. This innovation supports efficient feedstock assessment for biofuel development and genetic studies.

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Area of Science:

Agricultural Science
Biotechnology
Bioenergy

Background:

Assessing biomass composition is crucial for developing superior second-generation biofuel feedstocks.
Current robotic analysis systems are accurate but limited by slow, expensive large-scale sampling methods.
A high-throughput, cost-effective sampling process is needed for efficient biomass resource evaluation.

Purpose of the Study:

To develop a simple mechanical maize stalk core sampling device (CSD).
To enable collection of uniform samples compatible with robotic processing and analysis.
To achieve high-throughput sampling, collecting hundreds to thousands of samples daily.

Main Methods:

Development of a mechanical core sampling device (CSD) for maize stalks.
Testing the device's ability to collect uniform tissue cores.
Evaluating the throughput and sample quality for high-throughput analysis.

Main Results:

The CSD successfully collects uniform maize stalk samples suitable for robotic analysis.
A single operator using the CSD can collect over 1,000 samples in an eight-hour period.
Samples exhibit high homogeneity, minimal contamination, and preserved composition when stored appropriately.

Conclusions:

The CSD provides a repeatable and homogeneous stalk core sampling method.
The device offers significantly higher throughput than existing sampling techniques.
The CSD is expected to be valuable for bioenergy crops beyond maize, across various developmental stages.