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Related Experiment Videos

Polymorphism of avian sarcoma virus src proteins.

K Beemon, T Hunter, B M Sefton

    Journal of Virology
    |April 1, 1979
    PubMed
    Summary

    Avian sarcoma viruses exhibit significant genetic diversity in their p60src protein. This study reveals variations in molecular weight, antigenicity, and peptide structure among different viral strains, indicating substantial polymorphism.

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    Area of Science:

    • Molecular Virology
    • Oncogenesis
    • Protein Chemistry

    Background:

    • The src gene is a key oncogene in avian sarcoma viruses (ASVs).
    • The p60src protein is the product of the src gene and plays a crucial role in cell transformation.

    Purpose of the Study:

    • To compare the src gene products (p60src proteins) from seven different ASV strains.
    • To investigate the molecular, antigenic, and structural variability of p60src proteins.

    Main Methods:

    • In vitro translation of viral RNA to produce p60src.
    • Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to assess molecular weight.
    • Immunoprecipitation using specific antisera to evaluate antigenic properties.
    • Two-dimensional peptide mapping of methionine-containing tryptic peptides to analyze protein structure.

    Main Results:

    • Identified p60src protein from seven ASV strains, with apparent molecular weights ranging from 56,000 to 60,500.
    • Observed antigenic variability, with only three p60src proteins precipitating with specific antisera.
    • Peptide mapping revealed significant structural differences in p60src, both between and within viral strains, alongside four common peptides.

    Conclusions:

    • Significant polymorphism exists in the p60src proteins of avian sarcoma viruses.
    • Variations in p60src structure and antigenicity contribute to the diversity observed among ASV strains.

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