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Increasing SK2 channel activity impairs associative learning.

Bridget M McKay1, M Matthew Oh, Roberto Galvez

  • 1Department of Physiology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA.

Journal of Neurophysiology
|May 4, 2012
PubMed
Summary
This summary is machine-generated.

Pharmacologically reducing neuronal excitability in the hippocampus impairs learning. This supports the idea that reduced postburst afterhyperpolarization (AHP) in hippocampal neurons is a biomarker for successful associative learning.

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Area of Science:

  • Neuroscience
  • Cellular and Molecular Neuroscience

Background:

  • Enhanced intrinsic neuronal excitability in hippocampal pyramidal neurons, indicated by reduced postburst afterhyperpolarizations (AHPs), is a proposed biomarker for successful learning.
  • Pharmacological enhancement of neuronal excitability is known to facilitate learning, but the converse—whether reduced excitability hinders learning—remains underexplored.

Purpose of the Study:

  • To investigate if pharmacologically reducing hippocampal neuronal excitability can impede the acquisition of a hippocampus-dependent task.
  • To examine the role of small conductance potassium (SK) channels in associative learning.

Main Methods:

  • Focal application of the SK channel activator NS309 to the dorsal hippocampus in rats.
  • In vitro electrophysiological recordings of CA1 neurons to assess neuronal excitability.
  • In vivo assessment of spontaneous firing rates and trace eyeblink conditioning performance.
  • Measurement of SK2 channel mRNA and protein levels following conditioning.

Main Results:

  • NS309 increased the medium AHP and reduced action potential width in CA1 neurons in vitro.
  • In vivo, NS309 decreased the firing rate of CA1 pyramidal neurons and impaired trace eyeblink conditioning.
  • Trace eyeblink conditioning led to decreased hippocampal SK2 channel mRNA and protein levels.

Conclusions:

  • Modulation of SK channels is a critical cellular mechanism underlying associative learning.
  • Reductions in postburst AHP in hippocampal pyramidal neurons are further supported as a biomarker for successful learning.