Related Experiment Video
Updated: May 22, 2026

A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice
Published on: July 25, 2017
[Virulence genes and bacteriocins in Enterococcus faecalis strains isolated from different clinical samples in Maule
Carlos Padilla E1, María Núñez A, Andrés Padilla G
1Departamento de Microbiología, Universidad de Talca, Talca, Chile. cpadilla@utalca.cl
Abstract:
The presence of virulence genes (VG) and bacteriocins from different clinical samples was studied in Enterococcus faecalis isolated from urinary tract infections (UTI), bacteremia and endodontitis and was correlated with haemolysin and gelatinase activity. We evaluated the presence of VG by PCR in 150 strains of E. faecalis including cylA, aggA, efaA, eep, gelE, esp, as-48, bac31, entL50A/B, entA, entP, entB, enlA andentl071. Haemolysin and gelatinase activity was studied. gelE and cylA genes expressed hemolysin and gelatinase, respectively. This activity was observed in some strains of bacteremia, UTI and endodontitis. The highest number of VG was detected in bacteremic strains, being aggA and entA genes the most frequent. efaA, esp, entA, entL50A/B were associated with their clinical origin (p < 0.05). The most common genetic profile was aggA-eep-enlA-entL50A/B. E. faecalis from UTI, bacteremia and endodontitis presented different gene combinations. Some of the genes studied were related to their clinical origin. The results obtained in this study are similar to those reported in other countries.
Related Concept Videos
Clinical Significance of Antibiotic Resistance
Gene Regulation in Microbial Communities: Quorum Sensing
Regulation of Bacterial Virulence
Bacterial Gastroenteritis
Mechanism of Antibiotic Resistance in MRSA

