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Saccharomyces cerevisiae Exponential Growth Kinetics in Batch Culture to Analyze Respiratory and Fermentative Metabolism
Published on: September 30, 2018
Air-drying kinetics affect yeast membrane organization and survival.
Guillaume Lemetais1, Sébastien Dupont, Laurent Beney
1Université de Bourgogne/AGROSUP Dijon, UMR Procédés Alimentaires et Microbiologiques, 1 Esplanade Erasme, Dijon, France.
Applied Microbiology and Biotechnology
|May 4, 2012
Summary
Yeast cell survival during dehydration depends on plasma membrane (PM) reorganization. This study shows passive lateral rearrangements in the PM contribute to survival during desiccation stress.
Area of Science:
- Cell Biology
- Biophysics
Background:
- The plasma membrane (PM) is crucial for cellular survival under dehydration.
- Desiccation involves a transition from liquid to solid medium, posing significant environmental stress.
Purpose of the Study:
- To investigate the relationship between plasma membrane (PM) lateral organization and yeast survival during desiccation.
- To compare the effects of osmotic stress and air-drying on yeast strains.
Main Methods:
- Utilized wild-type and erg6Δ yeast strains.
- Observed PM lateral organization using Sur7-GFP fluorescent marker during progressive and rapid desiccation.
- Simulated air-drying using osmotic stress in liquid medium.
Main Results:
- Both wild-type and erg6Δ yeast strains exhibited similar survival responses to osmotic and desiccation stresses.
- Identical lethal dehydration magnitudes and kinetics were observed for both dehydration methods.
- Yeast survival after air-drying correlated with PM reorganization, indicating a role for passive membrane component rearrangements.
Conclusions:
- Plasma membrane (PM) reorganization, specifically passive lateral rearrangements, positively contributes to yeast survival during desiccation.
- Glycerol solutions effectively simulate air-drying desiccation conditions for experimental purposes.
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