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Modification of specific regions of the human cytomegalovirus genome during in vitro passage
1Centre de recherche en virologie, Institut Armand-Frappier, Laval-des-Rapides, Quebec.
Abstract:
Different regions of the human cytomegalovirus strain AD-169 DNA molecule were tested for stability using cloned Hind III fragments. Results indicate that insertion, deletion and/or rearrangement of nucleotide sequences occur at a relatively high frequency in all parts of the human cytomegalovirus genome during in vitro passage.
Insights
Human cytomegalovirus (HCMV) DNA sequences showed instability during laboratory passage. Genetic alterations like insertions and deletions were frequently observed across the entire HCMV genome.
Area of Science:
- Virology
- Molecular Biology
- Genetics
Background:
- Human cytomegalovirus (HCMV) is a significant human pathogen.
- Understanding the stability of the HCMV genome is crucial for research and therapeutic development.
Purpose of the Study:
- To assess the genetic stability of the human cytomegalovirus (HCMV) strain AD-169 genome.
- To identify regions prone to nucleotide sequence alterations during in vitro passage.
Main Methods:
- Cloning of specific Hind III fragments from the AD-169 strain DNA.
- Analysis of nucleotide sequences for insertions, deletions, and rearrangements.
Main Results:
- High-frequency occurrence of insertions, deletions, and/or rearrangements was detected.
- These genetic alterations were observed in all tested regions of the HCMV genome.
Conclusions:
- The human cytomegalovirus genome exhibits significant instability during in vitro passage.
- Frequent genetic modifications in HCMV DNA necessitate careful consideration in laboratory studies.
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