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Updated: May 22, 2026
Isolation and Identification of Limbal Niche Cells
Published on: October 27, 2023
Investigation of immunogenicity of cryopreserved limbal stem cells
Yan Zhang1, Cui-Xia Li, Li Sun
1Department of Ophthalmology, First Hospital of China Medical University, Shenyang 110001, Liaoning Province, China.
Aim:
To investigate changes in immunogenicity of cryopreserved limbal stem cells.
Methods:
Cryopreserved limbal stem cells, fresh primary limbal stem cells and blank controls were inoculated subcutaneously in C57BL-6 mice and the percentage of CD25 cells in limbal explants was determined by flow cytometry at day 21 post inoculation. Morphological studies were performed by light and electron microscopy of limbal explant sections.
Results:
The number of regional and systemic lymphocytes derived from cryopreserved limbal stem cells was lower than that from fresh primary limbal stem cells.
Conclusion:
Lymphocytes derived from cryopreserved limbal stem cells showed changes in immunogenicity, but the significance is unknown. The cryopreservation and thawing methods await further study.
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