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Synthesis and Evaluation of a Ruthenium-based Mitochondrial Calcium Uptake Inhibitor
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Published on: October 26, 2017

Ruthenium(II) complex-based fluorescent sensor for peroxynitrite.

Jingjin Ma1, Jiasheng Wu, Weimin Liu

  • 1Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, The Chinese Academy of Sciences, Beijing 100190, PR China.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|May 5, 2012
PubMed
Summary
This summary is machine-generated.

A new ruthenium complex, RuL, acts as a fluorescent sensor for detecting peroxynitrite (ONOO(-)). This sensor shows high selectivity and rapid response, quenching fluorescence upon ONOO(-) detection.

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The Synthesis, Characterization and Reactivity of a Series of Ruthenium N-triphosPh Complexes
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The Synthesis, Characterization and Reactivity of a Series of Ruthenium N-triphosPh Complexes

Published on: April 10, 2015

Area of Science:

  • Inorganic Chemistry
  • Analytical Chemistry
  • Biomedical Sensing

Background:

  • Peroxynitrite (ONOO(-)) is a key reactive nitrogen species implicated in oxidative stress and cellular damage.
  • Accurate detection of ONOO(-) is crucial for understanding its biological roles and developing therapeutic strategies.
  • Existing detection methods may lack selectivity, speed, or sensitivity.

Purpose of the Study:

  • To develop a novel fluorescent sensor for the selective and rapid detection of peroxynitrite (ONOO(-)).
  • To characterize the sensing properties of the new ruthenium complex, Ru(bpy)(2)[4-(2,2'-bipyridin-4-yloxy)phenol]Cl(2) (RuL).
  • To elucidate the sensing mechanism of RuL towards ONOO(-).

Main Methods:

  • Synthesis and characterization of the ruthenium complex RuL.
  • Fluorescence spectroscopy to monitor the interaction between RuL and peroxynitrite.
  • Testing selectivity against other reactive oxygen species (ROS) and reactive nitrogen species (RNS).
  • Kinetic studies to determine the reaction time.

Main Results:

  • The ruthenium complex RuL was successfully synthesized and demonstrated to be a fluorescent sensor.
  • Addition of ONOO(-) to aqueous RuL solutions caused distinct fluorescence quenching at 600nm.
  • RuL exhibited high selectivity for ONOO(-) over other ROS and RNS.
  • The sensing reaction occurred rapidly, with a response time of less than 1.5 seconds.

Conclusions:

  • The developed ruthenium complex RuL serves as an effective fluorescent sensor for peroxynitrite.
  • The sensor offers rapid and selective detection of ONOO(-), with potential applications in biological and chemical analysis.
  • The sensing mechanism involves an oxidative O-dealkylation reaction initiated by peroxynitrite.