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Estimation of sterols from dashmula.

N D Grampurohit1, M R Baichwal, C I Jolly

  • 1C.U. Shah College of Pharmacy, S.N.D.T. Women's University, Juhu Campus, Santacruz (W), Bombay - 400 949, India.

Ancient Science of Life
|May 5, 2012
PubMed
Summary

Dashmulakwatha, an Ayurvedic preparation, was analyzed for its phytosterol content. High-Performance Thin-Layer Chromatography (HPTLC) revealed a significant 7.165% W/W concentration of phytosterols, using beta-sitosterol as the reference standard.

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Area of Science:

  • Phytochemistry
  • Ayurvedic Medicine
  • Analytical Chemistry

Background:

  • Dashmula is a classical Ayurvedic formulation comprising ten roots.
  • Phytosterols are plant-derived compounds with potential health benefits.
  • Quantification of active compounds in Ayurvedic preparations ensures quality and efficacy.

Purpose of the Study:

  • To determine the phytosterol content in Dashmulakwatha.
  • To establish a quantitative analytical method for phytosterols in this Ayurvedic preparation.
  • To ensure the quality control of Dashmulakwatha.

Main Methods:

  • High-Performance Thin-Layer Chromatography (HPTLC) was employed for phytosterol analysis.
  • Beta-sitosterol was used as the reference standard for quantification.

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  • Dashmulakwatha preparation was analyzed according to established protocols.

    • Main Results:

    • The phytosterol content in Dashmulakwatha was quantified.
    • A concentration of 7.165% W/W phytosterols was found.
    • The results provide a quantitative baseline for phytosterol levels in Dashmulakwatha.

    Conclusions:

    • Dashmulakwatha contains a notable amount of phytosterols.
    • HPTLC is a suitable method for quantifying phytosterols in complex Ayurvedic formulations.
    • This study contributes to the standardization and quality assessment of Ayurvedic medicines.