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Colorimetric determination of reserpine in polyherbal formulations
M A Azan1, A Rahamathullah, P J Reddy
1J.S.S College of Pharmacy, Ootacamund - 643 001, Tamil Nadu, India.
Ancient Science of Life
|May 5, 2012
Summary
A new spectrophotometric method accurately estimates reserpine in polyherbal formulations. This method utilizes a reaction with 3-methylbenzolinone-2hydrazone (MBTH) to detect reserpine, offering a simple analytical approach.
Area of Science:
- Analytical Chemistry
- Pharmacognosy
- Spectrophotometry
Background:
- Reserpine is a key active compound in certain traditional medicines.
- Accurate quantification of reserpine in polyherbal formulations is crucial for quality control.
- Existing analytical methods may be complex or require specialized equipment.
Purpose of the Study:
- To develop a simple and reliable spectrophotometric method for reserpine estimation.
- To validate the method for use with polyherbal formulations.
- To provide an accessible analytical tool for quality assessment.
Main Methods:
- Spectrophotometric analysis using 3-methylbenzolinone-2hydrazone (MBTH) reagent.
- Formation of a violet-colored chromogen upon reaction with reserpine.
- Measurement of absorbance at 580 nm to quantify reserpine concentration.
Main Results:
- The developed method demonstrated a clear violet color formation.
- The chromogen exhibited maximum absorbance at 580 nm.
- The colored product remained stable for approximately 10 minutes, allowing for timely measurements.
Conclusions:
- A simple spectrophotometric method for reserpine estimation in polyherbal formulations has been successfully developed.
- The method relies on a colorimetric reaction with MBTH and cerric ammonium sulfate.
- This technique offers a straightforward approach for the quantitative analysis of reserpine.
