Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Ligand Binding and Linkage

Ligand Binding and Linkage

Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked. In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence the...

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

Proofreading

Proofreading

Synthesis of new DNA molecules is carried out by the enzyme DNA polymerase, which adds nucleotides on the daughter strand complementary to the template DNA strand. DNA polymerase has a higher affinity to add the correct base and ensures fidelity during DNA replication. Furthermore, it exhibits proofreading activity during replication, using an exonuclease domain that cuts off incorrect nucleotides from the nascent DNA strand.
Errors During Replication are Corrected by the DNA Polymerase Enzyme

Proofreading

Proofreading

Covalently Linked Protein Regulators

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.

Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

An integrative coding and non-coding SNPs analysis of the <i>CLDN-3</i> gene in humans to identify high-priority variants using <i>in-silico</i> analysis.

Frontiers in oncology·2026

Same author

Understanding of the different roles of Noggin in the Noggin-BMP-2 and Noggin-BMP-9 dimer complexes at the molecular level.

Scientific reports·2026

Same author

Endometrial tumorigenesis involves epigenetic plasticity demarcating non-coding somatic mutations and 3D-genome alterations.

Genome biology·2025

Same author

Network-Based Integrative Analysis to Identify Key Genes and Corresponding Reporter Biomolecules for Triple-Negative Breast Cancer.

Cancer medicine·2025

Same author

Investigating the potential of Juglans regia phytoconstituents for the treatment of cervical cancer utilizing network biology and molecular docking approach.

PloS one·2024

Same author

BM-MSC-Loaded Graphene-Collagen Cryogels Ameliorate Neuroinflammation in a Rat Spinal Cord Injury Model.

ACS applied bio materials·2024

Search research articles

Related Experiment Video

Updated: May 22, 2026

Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes

Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes

Published on: July 5, 2024

Cataloguing functionally relevant polymorphisms in gene DNA ligase I: a computational approach.

Abhishek A Singh, Dakshinamurthy Sivakumar, Pallavi Somvanshi

|May 5, 2012

Summary

This study computationally identifies functional single nucleotide polymorphisms (SNPs) in the LIG1 gene, crucial for DNA repair. Findings highlight specific SNPs that may alter protein function and influence drug response.

More Related Videos

Identification of Functional Protein Regions Through Chimeric Protein Construction

Identification of Functional Protein Regions Through Chimeric Protein Construction

Published on: January 8, 2019

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay (EMSA) and DNA-affinity Precipitation Assay (DAPA)

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay (EMSA) and DNA-affinity Precipitation Assay (DAPA)

Published on: August 21, 2016

Related Experiment Videos

Last Updated: May 22, 2026

Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes

Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes

Published on: July 5, 2024

Identification of Functional Protein Regions Through Chimeric Protein Construction

Identification of Functional Protein Regions Through Chimeric Protein Construction

Published on: January 8, 2019

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay (EMSA) and DNA-affinity Precipitation Assay (DAPA)

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay (EMSA) and DNA-affinity Precipitation Assay (DAPA)

Published on: August 21, 2016

Area of Science:

Genetics
Bioinformatics
Molecular Biology

Background:

The LIG1 gene is vital for DNA excision repair pathways.
Mutations in LIG1 can increase sensitivity to DNA damaging agents.
Understanding LIG1 genetic variations is key to predicting cellular responses.

Purpose of the Study:

To computationally identify functionally relevant single nucleotide polymorphisms (SNPs) in the human LIG1 gene.
To analyze the impact of these SNPs on protein function and gene regulation.
To provide insights into the relationship between LIG1 polymorphism and phenotype.

Main Methods:

Utilized multiple web servers (SIFT, PolyPhen, CUPSAT, FASTSNP, MAPPER) to analyze 792 reported LIG1 SNPs.
Assessed SNP impact on protein intolerance, damage, stability, splicing sites, and transcription factor binding.
Evaluated the structural and energetic effects of a specific damaging nsSNP (rs11666150).

Main Results:

Eleven nsSNPs were predicted as intolerant, thirteen as damaging, and two as destabilizing to protein structure.
The nsSNP rs11666150 was consistently predicted as damaging and showed increased overall energy in its mutant structure.
Twenty SNPs were identified in splicing modifier sites, and nine were predicted to affect transcription factor binding.

Conclusions:

The study successfully identified potentially functional SNPs in the LIG1 gene using a computational approach.
These findings offer crucial insights into how LIG1 polymorphisms may affect cellular phenotypes and drug responses.
The identified SNPs warrant further experimental validation for clinical relevance.