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Related Concept Videos

What is Gene Expression?01:42

What is Gene Expression?

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Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
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What is Gene Expression?01:36

What is Gene Expression?

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A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
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Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

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Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
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The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

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The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
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Expressing Solution Concentration02:48

Expressing Solution Concentration

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A solute is a component of a solution that is typically present at a much lower concentration than the solvent. Solute concentrations are often described with qualitative terms such as dilute (of relatively low concentration) and concentrated (of relatively high concentration).
Concentrations may be quantitatively assessed using a wide variety of measurement units, each convenient for particular applications. Molarity (M) is a useful concentration unit for many applications in chemistry.
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Cell Specific Gene Expression01:58

Cell Specific Gene Expression

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Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
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[Construction and expression of eukaryotic expression vector for pEGFP-GPC3].

Chun-hui Liu1, Zhi-ming Zhang, Jian-yong Liu

  • 1Department of Hepatobiliary Surgery, Guangxi Medical University, Nanning, China.

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology
|May 8, 2012
PubMed
Summary

Researchers successfully created and expressed the glypican-3 (GPC3) pEGFP-GPC3 eukaryotic expression vector in mouse dendritic cells (DCs). This advancement enables further study of GPC3 in immune cells.

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Area of Science:

  • Molecular Biology
  • Immunology

Context:

  • Glypican-3 (GPC3) is a cell surface heparan sulfate proteoglycan implicated in various biological processes, including cell growth and development.
  • Dendritic cells (DCs) are crucial antigen-presenting cells in the immune system, playing a key role in initiating adaptive immune responses.

Purpose:

  • To construct and validate a eukaryotic expression vector, pEGFP-GPC3, for the expression of glypican-3 (GPC3) in mouse dendritic cells (DCs).

Summary:

  • The GPC3 gene fragment was cloned into the pEGFP-N1 vector, creating the recombinant plasmid pEGFP-GPC3.
  • This vector was successfully transfected into mouse DCs using Lipofectamine 2000.
  • Expression of the GPC3-EGFP fusion protein was confirmed in DCs via fluorescent microscopy and Western blotting, with a detected protein size of 67 kDa.

Impact:

  • This study provides a functional eukaryotic expression vector for GPC3 in DCs, facilitating research into GPC3's role in immune cell function and potential therapeutic applications.
  • The successful expression demonstrates the utility of this vector system for studying GPC3 in an immunological context.