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Related Experiment Video

Updated: May 22, 2026

Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization

Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization

Published on: May 15, 2018

Solution-based targeted genomic enrichment for precious DNA samples.

Aiden Eliot Shearer¹, Michael S Hildebrand, Richard J H Smith

¹Department of Otolaryngology-Head & Neck Surgery, University of Iowa Carver College of Medicine, Iowa City, IA, 52242, USA.

BMC Biotechnology

|May 8, 2012

Summary

This summary is machine-generated.

We developed an improved solution-based targeted genomic enrichment (TGE) method for Illumina sequencing. This optimized protocol requires less DNA, increases yield, and enhances reproducibility for small labs.

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Area of Science:

Genomics
Molecular Biology
Biotechnology

Background:

Targeted genomic enrichment (TGE) enables selective sequencing of specific DNA regions.
Current TGE protocols can be time-consuming, require high DNA input, and lack reproducibility.
Improvements are needed to streamline workflows and increase efficiency.

Purpose of the Study:

To develop an optimized, solution-based TGE method for Illumina sequencing.
To address limitations of existing TGE protocols, focusing on efficiency and reproducibility.
To create a protocol suitable for non-automated, small- to medium-sized laboratory settings.

Main Methods:

A solution-based TGE workflow was developed for downstream Illumina sequencing.
Standard Illumina barcode indexes were incorporated during post-hybridization amplification for sample pooling.
The method employed Agilent SureSelect baits and reagents, with off-the-shelf library preparation components and custom oligonucleotide adaptors.

Main Results:

The developed method allows for sample pooling prior to sequencing.
It utilizes commercially available reagents and components for library preparation and sequencing adaptors.
The protocol is designed for a non-automated workflow.

Conclusions:

The solution-based TGE method is optimized for small- and medium-sized laboratories.
It effectively reduces required DNA input and increases capture yield.
The protocol enhances efficiency and reproducibility compared to standard methods.