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Updated: May 22, 2026

Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers
Published on: March 4, 2021
Ion mobility separation of variant histone tails extending to the "middle-down" range
Alexandre A Shvartsburg1, Yupeng Zheng, Richard D Smith
1Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99352, United States. alexandre.shvartsburg@pnl.gov
Abstract:
Differential ion mobility spectrometry (FAIMS) can baseline-resolve multiple variants of post-translationally modified peptides extending to the 3-4 kDa range, which differ in the localization of a PTM as small as acetylation. Essentially orthogonal separations for different charge states expand the total peak capacity with the number of observed states that increases for longer polypeptides. This potentially enables resolving localization variants for yet larger peptides and even intact proteins.
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