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Related Concept Videos

Satellite Stem Cells and Muscular Dystrophy01:21

Satellite Stem Cells and Muscular Dystrophy

Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...
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Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
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Hypertrophy is the increase in the size of individual cells, resulting in the enlargement of a tissue or organ. Unlike hyperplasia, which involves an increase in cell number, hypertrophy is characterized by an increase in cell volume. This process often occurs in response to higher functional demand or hormonal stimulation, leading to the production of more structural proteins and organelles, thereby enhancing the cells' work capacity.There are two primary types of hypertrophy: physiological...
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Updated: May 22, 2026

Isolation of Quiescent Stem Cell Populations from Individual Skeletal Muscles
11:35

Isolation of Quiescent Stem Cell Populations from Individual Skeletal Muscles

Published on: December 9, 2022

Short-term calorie restriction enhances skeletal muscle stem cell function.

Massimiliano Cerletti1, Young C Jang, Lydia W S Finley

  • 1Department of Stem Cell and Regenerative Biology, Harvard University, Harvard Stem Cell Institute, 7 Divinity Avenue, Cambridge, MA 02138, USA.

Cell Stem Cell
|May 8, 2012
PubMed
Summary
This summary is machine-generated.

Calorie restriction (CR) enhances stem cell function, boosting muscle repair and regeneration. This metabolic intervention improves tissue recovery and cell transplantation success in both young and old mice.

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Area of Science:

  • Gerontology
  • Stem Cell Biology
  • Metabolic Regulation

Background:

  • Calorie restriction (CR) is known to extend lifespan and reduce age-related diseases.
  • The precise mechanisms by which CR exerts these beneficial effects, particularly on tissue regeneration and stem cell function, are not fully understood.

Purpose of the Study:

  • To investigate the role of CR in modulating tissue-specific stem cell function.
  • To elucidate the impact of CR on skeletal muscle repair and regeneration processes.
  • To determine if CR influences the efficacy of stem cell transplantation.

Main Methods:

  • Utilized mouse skeletal muscle as a model system.
  • Assessed stem cell availability and activity under CR conditions in young and old animals.
  • Quantified mitochondrial abundance and expression of metabolic/longevity regulators.
  • Evaluated endogenous muscle repair capacity post-CR.
  • Performed muscle stem cell transplantation experiments with CR-treated donor and/or recipient animals.

Main Results:

  • Short-term CR significantly increased stem cell availability and activity in skeletal muscle.
  • CR led to increased mitochondrial abundance and induced key metabolic and longevity regulators.
  • CR enhanced endogenous muscle repair capabilities.
  • CR initiated in either donor or recipient mice improved the engraftment and contribution of transplanted stem cells to muscle regeneration.

Conclusions:

  • Metabolic factors, influenced by CR, play a crucial role in regulating stem cell function.
  • Enhanced stem cell function under CR contributes to improved tissue repair and recovery from injury.
  • CR can positively impact the success of stem cell transplantation by modulating the stem cell niche and function.