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Updated: May 22, 2026
A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
Published on: November 23, 2015
Ag/Au bi-metallic film based color surface plasmon resonance biosensor with enhanced sensitivity, color contrast and
Chung-Tien Li1, Kun-Chi Lo, Hsin-Yun Chang
1Department of Materials Science and Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan ROC.
Abstract:
In wavelength surface plasmon resonance (SPR) biosensor, the manipulation of SPR dispersion relation by Ag/Au bi-metallic film was first time implemented. Due to the enhanced resonant wavelength shift and the sharper SPR slope of using Ag/Au bi-metallic film, the illuminated color of reflection shows one order of magnitude greater contrast than conventional SPR biosensors. Such an Ag/Au bi-metallic film based color SPR biosensor (CSPRB) allows the detail bio-interactions, for example 100 nM streptavidin, to be distinguished by directly observing the color change of reflection through naked eyes rather than the analysis of spectrometer. In addition to the enhanced sensitivity and color contrast, this CSPRB also possesses a great linear detection range up to 0.0254 RIU, which leading to the application of point-of-care tests.
