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Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
Published on: May 8, 2013
High-resolution melting analysis for rapid detection of linezolid resistance (mediated by G2576T mutation) in
Emma M Gabriel1, Pierre E Douarre, Siobhan Fitzgibbon
1Cork Institute of Technology, Cork, Ireland.
Journal of Microbiological Methods
|May 8, 2012
Abstract:
We describe a novel HRM-PCR (high-resolution melting) assay capable of the accurate identification of the G2576T point mutation in domain V of the 23S rRNA genes attributed to linezolid resistance in Staphylococcus epidermidis. This rapid method demonstrated 100% correlation with the previously established NheI restriction digest assay.