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Preparation of Single-cell Suspensions for Cytofluorimetric Analysis from Different Mouse Skin Regions
Published on: April 20, 2016
The skin, a novel niche for recirculating B cells
Skye A Geherin1, Sarah R Fintushel, Michael H Lee
1Department of Pathobiology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, PA 19104, USA.
Journal of Immunology (Baltimore, Md. : 1950)
|May 8, 2012
Summary
Skin B cells are distinct and play a key role in chronic inflammatory skin diseases. These immune cells accumulate in inflamed skin, potentially enhancing immune responses and autoimmunity.
Area of Science:
- Immunology
- Dermatology
- Cell Biology
Background:
- B cells are implicated in chronic inflammatory skin diseases but remain poorly understood.
- Characterizing skin-associated B cells is crucial for understanding their role in disease pathogenesis.
Purpose of the Study:
- To investigate the characteristics and behavior of B cells within inflamed skin.
- To elucidate the homing mechanisms and functional properties of skin-associated B cells.
Main Methods:
- Ovine model of granulomatous skin inflammation.
- Flow cytometry and immunohistochemistry to analyze B cell populations.
- Chemokine receptor ligand binding assays.
Main Results:
- B cells increase in inflamed skin and draining lymph, forming a distinct population from lymph node B cells.
- Skin B cells exhibit increased expression of MHC class II, CD1, and CD80/86, suggesting enhanced antigen-presenting capacity.
- Skin B cells utilize CCR6-CCL20 for migration, indicating a unique homing mechanism.
Conclusions:
- Skin B cells are a heterogeneous population with distinct functional and migratory properties.
- These cells may play a significant role in local immune responses and inflammation within the skin.
- Understanding skin B cell dynamics offers new insights into autoimmune and infectious skin diseases.
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