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Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids

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Flow cytometry for the development of biotechnological processes with microalgae.

P Hyka¹, S Lickova, P Přibyl

¹Institute of Biotechnology, Zurich University of Applied Sciences (ZHAW), Campus Grüental, CH-8820 Wädenswil, Switzerland.

Biotechnology Advances

|May 8, 2012

Summary

Flow cytometry (FCM) analyzes microalgal cell properties for optimizing biofuel production. This method aids in characterizing microalgal behavior for efficient biotechnological applications.

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Area of Science:

Biotechnology
Microalgal Research
Sustainable Energy

Background:

Microalgae are explored as sustainable sources for biofuels and valuable compounds.
Optimizing microalgal production requires understanding species behavior under various conditions.

Purpose of the Study:

To review the application of flow cytometry (FCM) in microalgal biotechnology.
To highlight FCM's potential in developing efficient microalgal bioprocesses.

Main Methods:

Flow cytometry (FCM) analyzes intrinsic cellular features like autofluorescence and size.
FCM can count or sort cells based on desired physiological or morphological traits.
Methods involve intrinsic cell features, with or without fluorescent staining.

Main Results:

FCM is valuable for analyzing microalgal physiological states.
FCM aids in characterizing microalgal behavior for bioprocess optimization.
FCM enables analysis of biomass composition, enzyme activity, and cell viability.

Conclusions:

Flow cytometry (FCM) is a powerful tool for advancing microalgal biotechnology.
FCM facilitates the isolation of high-value microalgal strains.
FCM contributes to the rapid development of feasible microalgal bioprocesses for sustainable production.