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iChip

iChip

The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...

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Related Experiment Video

Updated: May 22, 2026

Detection of a CDH1 Rare Transcript Variant in Fresh-frozen Gastric Cancer Tissues by Chip-based Digital PCR

Detection of a CDH1 Rare Transcript Variant in Fresh-frozen Gastric Cancer Tissues by Chip-based Digital PCR

Published on: February 5, 2018

HPV 9G DNAChip: Based on the 9G DNAChip technology.

Keum-Soo Song¹, Satish Balasaheb Nimse, Heejung An

¹Biometrix Technology, Inc. 202 BioVenture Plaza, Chuncheon 200-161, Republic of Korea.

Journal of Virological Methods

|May 8, 2012

Summary

The novel HPV 9G DNAChip accurately detects and differentiates HPV genotypes in clinical samples. This diagnostic tool shows 100% agreement with sequencing, offering precise human papillomavirus genotyping.

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Using a Pan-Viral Microarray Assay (Virochip) to Screen Clinical Samples for Viral Pathogens

Published on: April 27, 2011

Related Experiment Videos

Last Updated: May 22, 2026

Detection of a CDH1 Rare Transcript Variant in Fresh-frozen Gastric Cancer Tissues by Chip-based Digital PCR

Detection of a CDH1 Rare Transcript Variant in Fresh-frozen Gastric Cancer Tissues by Chip-based Digital PCR

Published on: February 5, 2018

Chromogenic In Situ Hybridization as a Tool for HPV-Related Head and Neck Cancer Diagnosis

Chromogenic In Situ Hybridization as a Tool for HPV-Related Head and Neck Cancer Diagnosis

Published on: June 14, 2019

Using a Pan-Viral Microarray Assay (Virochip) to Screen Clinical Samples for Viral Pathogens

Using a Pan-Viral Microarray Assay (Virochip) to Screen Clinical Samples for Viral Pathogens

Published on: April 27, 2011

Area of Science:

Molecular Diagnostics
Virology
Genomics

Background:

Human Papillomavirus (HPV) infection is a major cause of cervical cancer.
Accurate HPV genotyping is crucial for effective patient management and prevention strategies.
Existing diagnostic methods can be time-consuming or lack comprehensive genotype coverage.

Purpose of the Study:

To develop and evaluate the HPV 9G DNAChip for sensitive and specific detection of HPV genotypes.
To assess the performance of the HPV 9G DNAChip against established sequencing methods.
To determine the diagnostic accuracy of the HPV 9G DNAChip for clinical samples.

Main Methods:

Development of the HPV 9G DNAChip assay for HPV detection.
Optimization of hybridization and washing conditions (30 min hybridization, 2x2 min washing at 25°C).
Comparative analysis of HPV 9G DNAChip results with sequencing for 959 HPV-positive and 82 HPV-negative clinical samples.

Main Results:

The HPV 9G DNAChip demonstrated a high signal-to-background ratio (SBR) of 50-70.
Achieved 100% target-specific hybridization under optimized conditions.
Showed 100% agreement with sequencing for both HPV-positive and HPV-negative samples.
Successfully discriminated 19 different HPV genotypes in positive samples.

Conclusions:

The HPV 9G DNAChip is a highly accurate tool for HPV genotyping.
Its performance characteristics, including high SBR and specificity, make it suitable for clinical diagnostics.
The assay's efficiency and accuracy at low temperatures offer a promising advancement in HPV detection.