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Reversing bladder outlet obstruction attenuates systemic and tissue oxidative stress
Wei-Yu Lin1, Shi-Bei Wu, Yi-Pai Lin
1Division of Urology, Department of Surgery, Chang Gung Memorial Hospital, Chia-Yi, Taiwan.
BJU International
|May 9, 2012
Summary
Partial bladder outlet obstruction (PBOO) elevates oxidative stress in bladder tissue and systemically. Reversing PBOO progressively reduces this oxidative stress, offering a potential therapeutic target.
Area of Science:
- Urology
- Biochemistry
- Nephrology
Background:
- Partial bladder outlet obstruction (PBOO) is associated with oxidative damage in bladder tissue.
- Systemic oxidative stress markers are also elevated in conditions of PBOO.
Purpose of the Study:
- To determine if PBOO increases systemic oxidative stress.
- To investigate if relieving PBOO can reduce this stress.
Main Methods:
- Male rabbits underwent surgical PBOO for 4 weeks, with relief in separate groups assessed at 4 and 8 weeks.
- Oxidative stress biomarkers measured included 8-hydroxy-2'-deoxyguanosine (8-OHdG), malondialdehyde (MDA), total antioxidant capacity (TAC), and glutathione (GSH).
- Bladder tissue mitochondrial DNA copy number and 8-OHdG content were also analyzed.
Main Results:
- PBOO significantly increased urinary and plasma 8-OHdG, plasma MDA, and bladder tissue 8-OHdG.
- PBOO led to significant decreases in TAC, GSH, and bladder mitochondrial DNA copy number.
- Relief of PBOO resulted in all measured values returning towards control levels by 8 weeks.
Conclusions:
- Partial bladder outlet obstruction significantly increases both systemic and tissue oxidative stress.
- Reversal of PBOO leads to a progressive reduction in systemic and tissue oxidative stress.
