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IbeB is involved in the invasion and pathogenicity of avian pathogenic Escherichia coli

Shaohui Wang1, Zhenyu Shi, Yongjie Xia

  • 1Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China. shwang0827@126.com

Veterinary Microbiology
|May 9, 2012
PubMed

Insights

The ibeB gene is conserved in E. coli and crucial for avian pathogenic E. coli (APEC) virulence. Loss of ibeB reduces invasion and colonization in animal models, impacting APEC pathogenicity.

Area of Science:

  • Microbiology
  • Pathogenesis
  • Genetics

Background:

  • The ibeB gene aids neonatal meningitis Escherichia coli (NMEC) in penetrating human brain microvascular endothelial cells (HBMECs).
  • The role of ibeB in avian pathogenic E. coli (APEC) pathogenicity is not well understood.

Purpose of the Study:

  • Investigate the distribution of the ibeB gene in Chinese APEC strains.
  • Determine the involvement of IbeB in APEC pathogenicity.

Main Methods:

  • Screened 100 E. coli isolates for the ibeB gene.
  • Constructed and characterized an ibeB mutant of APEC strain DE205B.
  • Evaluated invasion capacity in cell culture and virulence in animal models.

Main Results:

  • The ibeB gene was present in all 100 APEC isolates with high sequence homology, indicating conservation.
  • The ibeB mutant showed reduced invasion of DF-1 cells and impaired virulence in animal models.
  • Loss of ibeB decreased colonization and invasion in the brains and lungs of infected animals, with partial recovery upon complementation.

Conclusions:

  • IbeB is a conserved gene in E. coli, irrespective of pathotype.
  • IbeB plays a significant role in APEC invasion and pathogenicity.
  • Reduced expression of invasion-associated genes like ibeA and ompA contributes to the virulence defect in ibeB mutants.

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