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Related Experiment Video

Updated: May 22, 2026

Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR
11:37

Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR

Published on: June 11, 2016

Pyrosequencing reveals bacteria carried in different wind-eroded sediments.

Terrence Gardner1, Veronica Acosta-Martinez, Francisco J Calderón

  • 1USDA-ARS, Wind Erosion &Water Conservation Research Unit, Lubbock, TX 79415, USA. terrence.gardner@ars.usda.gov

Journal of Environmental Quality
|May 9, 2012
PubMed
Summary

Wind erosion disperses soil microbes, with coarser sediments carrying more diverse bacteria than fine dust. This loss of microorganisms impacts soil health and function.

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Pyrosequencing for Microbial Identification and Characterization
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Pyrosequencing for Microbial Identification and Characterization
12:37

Pyrosequencing for Microbial Identification and Characterization

Published on: August 22, 2013

Area of Science:

  • Microbial ecology
  • Soil science
  • Environmental science

Background:

  • Wind erosion's impact on soil microbial communities remains largely uncharacterized.
  • Pyrosequencing offers advanced capabilities for analyzing microbial diversity in soils and eroded sediments.
  • Understanding microbial losses due to wind erosion is crucial for soil sustainability.

Purpose of the Study:

  • To evaluate bacterial diversity in wind-eroded sediments from different organic-rich soils.
  • To compare microbial communities in coarse versus fine wind-eroded sediment fractions.
  • To assess the implications of microbial loss via wind erosion on soil functioning.

Main Methods:

  • Utilized a portable field wind tunnel to collect wind-eroded sediments.
  • Analyzed bacterial diversity using pyrosequencing.
  • Characterized sediment particle size fractions (coarse >106 μm, fine <106 μm).
  • Employed infrared spectroscopy to analyze soil carbon composition.

Main Results:

  • Bacteria in eroded sediments reflect source soil composition, with variations based on soil properties.
  • Coarse wind-eroded sediment generally exhibited higher bacterial diversity than fine dust.
  • Specific bacterial groups were found to be more abundant in either fine dust or coarse sediment, indicating distinct soil niches.
  • Wind erosion preferentially removes labile organic matter and associated microorganisms.

Conclusions:

  • Wind-eroded sediments act as fingerprints of the source soil's microbial community.
  • Differential distribution of bacteria in soil particles affects microbial communities in eroded sediments.
  • The loss of active, labile organic matter and microorganisms via wind erosion negatively impacts soil quality and function.
  • Understanding these microbial dynamics is vital for maintaining soil health and ecosystem services.